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Functional Characterization and Protein Engineering of a Triterpene 3-/6-/2'-O-Glycosyltransferase Reveal a Conserved Residue Critical for the Regiospecificity.
Zhang, Meng; Yi, Yang; Gao, Bai-Han; Su, Hui-Fei; Bao, Yang-Oujie; Shi, Xiao-Meng; Wang, Hai-Dong; Li, Fu-Dong; Ye, Min; Qiao, Xue.
Affiliation
  • Zhang M; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
  • Yi Y; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
  • Gao BH; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
  • Su HF; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
  • Bao YO; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
  • Shi XM; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
  • Wang HD; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
  • Li FD; National Science Center for Physical Sciences at Microscale, Division of Molecular & Cell Biophysics and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
  • Ye M; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
  • Qiao X; State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
Angew Chem Int Ed Engl ; 61(8): e202113587, 2022 02 14.
Article in En | MEDLINE | ID: mdl-34894044
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Full text: 1 Database: MEDLINE Main subject: Saponins / Triterpenes / Protein Engineering / Glycosyltransferases Language: En Journal: Angew Chem Int Ed Engl Year: 2022 Type: Article Affiliation country: China
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Full text: 1 Database: MEDLINE Main subject: Saponins / Triterpenes / Protein Engineering / Glycosyltransferases Language: En Journal: Angew Chem Int Ed Engl Year: 2022 Type: Article Affiliation country: China