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Identification of allergens and allergen hydrolysates by proteomics and metabolomics: A comparative study of natural and enzymolytic bee pollen.
Tao, Yuxiao; Yin, Shuting; Fu, Linglin; Wang, Miao; Meng, Lifeng; Li, Fukai; Xue, Xiaofeng; Wu, Liming; Li, Qiangqiang.
Affiliation
  • Tao Y; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100093, China.
  • Yin S; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100093, China.
  • Fu L; Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, China.
  • Wang M; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100093, China.
  • Meng L; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100093, China.
  • Li F; Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.
  • Xue X; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100093, China.
  • Wu L; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100093, China. Electronic address: apiswu@126.com.
  • Li Q; Institute of Apicultural Research, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100093, China. Electronic address: qiangqiangli1991@163.com.
Food Res Int ; 158: 111572, 2022 08.
Article in En | MEDLINE | ID: mdl-35840258
Bee pollen as a plant-derived food is consumed as nutritional/functional supplements by humans. But it might confer foodborne allergenicity in susceptible populations, limiting its extensive application. In this study, five potential allergens including profilin, cystatin, prolamin, expansin, and alcohol dehydrogenase in bee pollen derived from Brassica campestris (BP-Bc), were identified through mass spectrometry-based proteomic analysis. Moreover, different types of enzymes (cellulases, pectases, and papains) serve biological roles in pollen wall breaking and expansion, but also promote allergen release and degradation. Proteomic analysis showed that profilin, cystatin, and alcohol dehydrogenase were significantly reduced in BP-Bc following joint treatment with three enzymes. Metabolomic characterization of potential enzymatic hydrolysates of these significantly-decreased allergens was performed, which showed nine major oligopeptides and six amino acids at significantly higher levels in the enzyme-treated BP-Bc. These findings clarified the culprit responsible for bee pollen allergy and the mechanism of enzymatic desensitization for its further development.
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Full text: 1 Database: MEDLINE Main subject: Allergens / Food Hypersensitivity Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: Food Res Int Year: 2022 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Allergens / Food Hypersensitivity Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: Food Res Int Year: 2022 Type: Article Affiliation country: China