Pinus massoniana needle extracts attenuate oxidative stress injury in cerebral ischemia reperfusion rats by regulating JNK3/caspase-3 signal transduction.
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 51(5): 563-572, 2022 Nov 25.
Article
in En
| MEDLINE
| ID: mdl-36581582
ABSTRACT
OBJECTIVE:
To investigate the effect and mechanism of Pinus massoniana needle extracts (PNE) on oxidative stress injury in cerebral ischemia reperfusion rats.METHODS:
The SD male rats were randomly divided into sham group, model control group, Edaravone (3â mg/kg) group, PNE low-dose (200â mg/kg), medium-dose (400â mg/kg) and high-dose (800â mg/kg) groups. PNE was administered by gavage for 7â d before modeling and 6â h after modeling in PNE treatment groups; Edaravone was given by intraperitoneal injection 7â d before modeling and 6â h after reperfusion. The rat model of cerebral ischemia reperfusion injury was established by middle cerebral artery occlusion method. After 24â h of reperfusion, the neurological deficit score, brain water content and cerebral infarction volume of rats were measured. The pathological changes of cerebral cortex and hippocampus were observed by HE staining, and the number of normal nerve cells was counted. The apoptosis rate of neurons in cerebral cortex was detected by TUNEL method. The content of nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) activity in ischemic brain tissue were detected. The protein expression of c-Jun N-terminal kinase (JNK) 3, phosphorylated JNK3 (p-JNK3), B-cell lymphoma protein(Bcl) -2, Bcl-2 associated X (Bax), cytochrome C and caspase-3 in cerebral cortex were detected by Western blotting method.RESULTS:
Compared with the model control group, the behavioral score, brain water content and cerebral infarction volume in PNE groups were significantly reduced (all P<0.05), the pathological damage of cerebral cortex and hippocampal CA1 area was significantly alleviated, and the number of normal nerve cells in ischemic cortex and hippocampal CA1 area was increased (all P<0.05). The medium-dose PNE group had the best effect. Compared with the model control group, the apoptosis rate of cortical neurons, the content of NO and MDA in cerebral cortex, the ratio of p-JNK3/JNK3, the expression level of cytochrome C and caspase-3 protein in PNE medium-dose group were significantly reduced , and the activity of SOD, the Bcl-2/Bax ratio were significantly improved (all P<0.05).CONCLUSION:
PNE ameliorates brain injury after cerebral ischemia reperfusion in rats, which may be related to scavenging NO and MDA, inhibiting oxidative stress-mediated JNK3/caspase-3 signsal transduction to inhibit neuronal apoptosis.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Plant Extracts
/
Reperfusion Injury
/
Brain Ischemia
/
Oxidative Stress
Type of study:
Prognostic_studies
Language:
En
Journal:
Zhejiang Da Xue Xue Bao Yi Xue Ban
Year:
2022
Type:
Article
Affiliation country:
China