Convenient tumor 3D spheroid arrays manufacturing via acoustic excited bubbles for in situ drug screening.
Lab Chip
; 23(6): 1593-1602, 2023 03 14.
Article
in En
| MEDLINE
| ID: mdl-36752157
ABSTRACT
The quick and convenient fabrication of in vitro tumor spheroids models has been pursued for clinical drug discovery and personalized therapy. Here, uniform three-dimensional (3D) tumor spheroids are quickly constructed by acoustically excited bubble arrays in a microfluidic chip and performed drug response testing in situ. In detail, bubble oscillation excited by acoustic waves induces second radiation force, resulting in the cells rotating and aggregating into tumor spheroids, which obtain controllable sizes ranging from 30 to 300 µm. These spherical tumor models are located in microfluidic networks, where drug solutions with gradient concentrations are generated from 0 to 18 mg mL-1, so that the cell spheroids response to drugs can be monitored conveniently and efficiently. This one-step tumor spheroids manufacturing method significantly reduces the model construction time to less than 15 s and increases efficiency by eliminating additional transfer processes. These significant advantages of convenience and high-throughput manufacturing make the tumor models promising for use in tumor treatment and point-of-care diagnosis.
Full text:
1
Database:
MEDLINE
Main subject:
Microfluidics
/
Drug Discovery
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
En
Journal:
Lab Chip
Year:
2023
Type:
Article
Affiliation country:
China