Your browser doesn't support javascript.
loading
Convenient tumor 3D spheroid arrays manufacturing via acoustic excited bubbles for in situ drug screening.
Zheng, Jingjing; Hu, Xuejia; Gao, Xiaoqi; Liu, Yantong; Zhao, Shukun; Chen, Longfei; He, Guoqing; Zhang, Jingwei; Wei, Lei; Yang, Yi.
Affiliation
  • Zheng J; School of Physics & Technology, Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, Department of Clinical Laboratory, Institute of Medicine and Physics, Renmin Hospital, Wuhan University, Wuhan 430072, China. yangyiys@whu.edu.cn.
  • Hu X; Shenzhen Research Institute, Wuhan University, Shenzhen 518000, China.
  • Gao X; Department of Electronic Engineering, School of Electronic Science and Engineering, Xiamen University, Xiamen 361005, China.
  • Liu Y; School of Physics & Technology, Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, Department of Clinical Laboratory, Institute of Medicine and Physics, Renmin Hospital, Wuhan University, Wuhan 430072, China. yangyiys@whu.edu.cn.
  • Zhao S; Shenzhen Research Institute, Wuhan University, Shenzhen 518000, China.
  • Chen L; School of Physics & Technology, Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, Department of Clinical Laboratory, Institute of Medicine and Physics, Renmin Hospital, Wuhan University, Wuhan 430072, China. yangyiys@whu.edu.cn.
  • He G; Shenzhen Research Institute, Wuhan University, Shenzhen 518000, China.
  • Zhang J; School of Physics & Technology, Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, Department of Clinical Laboratory, Institute of Medicine and Physics, Renmin Hospital, Wuhan University, Wuhan 430072, China. yangyiys@whu.edu.cn.
  • Wei L; Shenzhen Research Institute, Wuhan University, Shenzhen 518000, China.
  • Yang Y; School of Physics & Technology, Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, Department of Clinical Laboratory, Institute of Medicine and Physics, Renmin Hospital, Wuhan University, Wuhan 430072, China. yangyiys@whu.edu.cn.
Lab Chip ; 23(6): 1593-1602, 2023 03 14.
Article in En | MEDLINE | ID: mdl-36752157
ABSTRACT
The quick and convenient fabrication of in vitro tumor spheroids models has been pursued for clinical drug discovery and personalized therapy. Here, uniform three-dimensional (3D) tumor spheroids are quickly constructed by acoustically excited bubble arrays in a microfluidic chip and performed drug response testing in situ. In detail, bubble oscillation excited by acoustic waves induces second radiation force, resulting in the cells rotating and aggregating into tumor spheroids, which obtain controllable sizes ranging from 30 to 300 µm. These spherical tumor models are located in microfluidic networks, where drug solutions with gradient concentrations are generated from 0 to 18 mg mL-1, so that the cell spheroids response to drugs can be monitored conveniently and efficiently. This one-step tumor spheroids manufacturing method significantly reduces the model construction time to less than 15 s and increases efficiency by eliminating additional transfer processes. These significant advantages of convenience and high-throughput manufacturing make the tumor models promising for use in tumor treatment and point-of-care diagnosis.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Microfluidics / Drug Discovery Type of study: Diagnostic_studies / Screening_studies Language: En Journal: Lab Chip Year: 2023 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Microfluidics / Drug Discovery Type of study: Diagnostic_studies / Screening_studies Language: En Journal: Lab Chip Year: 2023 Type: Article Affiliation country: China