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GFAP-expressing cells in the adult hypothalamus can generate multiple neural cell lineages in vitro.
Butruille, Lucile; Batailler, Martine; Vaudin, Pascal; Pillon, Delphine; Migaud, Martine.
Affiliation
  • Butruille L; CNRS, IFCE, INRAE, Université de Tours, PRC, F-37380 Nouzilly, France.
  • Batailler M; CNRS, IFCE, INRAE, Université de Tours, PRC, F-37380 Nouzilly, France.
  • Vaudin P; CNRS, IFCE, INRAE, Université de Tours, PRC, F-37380 Nouzilly, France.
  • Pillon D; CNRS, IFCE, INRAE, Université de Tours, PRC, F-37380 Nouzilly, France.
  • Migaud M; CNRS, IFCE, INRAE, Université de Tours, PRC, F-37380 Nouzilly, France. Electronic address: Martine.Migaud@inrae.fr.
Neurosci Lett ; 824: 137674, 2024 Feb 28.
Article in En | MEDLINE | ID: mdl-38355005
ABSTRACT
Adult neural stem cells (NSCs) located in the two canonical neurogenic niches, the subventricular zone (SVZ) and the subgranular zone (SGZ), express the glial fibrillary acidic protein (GFAP). Recently, proliferative activity has been described in the hypothalamus although the characterization of hypothalamic neural stem/progenitor cells (NSPCs) is still uncertain. We therefore investigated whether hypothalamic GFAP-positive cells, as in the SVZ and SGZ, also have neurogenic potential. We used a transgenic mouse line expressing green fluorescent protein (GFP) under the control of the GFAP promoter. GFAP-GFP expressing cells are localized in the ependymal layer as well as in the parenchyma of the mediobasal hypothalamus (MBH) and express Sox2, a marker for NSCs. Interestingly, no sexual dimorphism was observed in the numbers of GFP + and GFP-Sox2 + cells. After cells sorting, these cells were able to generate neurospheres in vitro and give rise to neurons, astrocytes and oligodendrocytes. Taken together, these results show that hypothalamic GFAP-expressing cells form a population of NSPCs.
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Full text: 1 Database: MEDLINE Main subject: Neural Stem Cells Language: En Journal: Neurosci Lett Year: 2024 Type: Article Affiliation country: France

Full text: 1 Database: MEDLINE Main subject: Neural Stem Cells Language: En Journal: Neurosci Lett Year: 2024 Type: Article Affiliation country: France