Investigations of Limeum Indicum Plant for Diabetes Mellitus and Alzheimer's Disease Dual Therapy: Phytochemical, GC-MS Chemical Profiling, Enzyme Inhibition, Molecular Docking and In-Vivo Studies.
Chem Biodivers
; 21(6): e202301858, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38608202
ABSTRACT
Limeum indicum has been widely utilized in traditional medicine but no experimental work has been done on this herb. The primary objective of this study was to conduct a phytochemical analysis and assess the multifunctional capabilities of aforementioned plant in dual therapy for Alzheimer's disease (AD) and Typeâ
2 diabetes (T2D). The phytochemical screening of ethanol, methanol extract, and their derived fractions of Limeum indicum was conducted using GC-MS, HPLC, UV-analysis and FTIR. The antioxidant capacity was evaluated by DPPH method. The inhibitory potential of the extracts/fractions against α-, ß-glucosidase acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and monoaminine oxidases (MAO-A & B) was evaluated. Results revealed that acetonitrile fraction has highest inhibitory potential against α-glucosidase (IC50=68.47±0.05â
µg/mL), methanol extract against ß-glucosidase (IC50=91.12±0.07â
µg/mL), ethyl acetate fraction against AChE (IC50=59.0±0.02â
µg/mL), ethanol extract against BChE (28.41±0.01â
µg/mL), n-hexane fraction against MAO-A (IC50=150.5±0.31â
µg/mL) and methanol extract for MAO-B (IC50=75.95±0.13â
µg/mL). The docking analysis of extracts\fractions suggested the best binding scores within the active pocket of the respective enzymes. During the in-vivo investigation, ethanol extract produced hypoglycemic effect (134.52±2.79 and 119.38±1.40â
mg/dl) after 21â
days treatment at dose level of 250 and 500â
mg/Kg. Histopathological findings further supported the in-vivo studies.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Acetylcholinesterase
/
Butyrylcholinesterase
/
Plant Extracts
/
Alzheimer Disease
/
Molecular Docking Simulation
/
Phytochemicals
/
Hypoglycemic Agents
/
Gas Chromatography-Mass Spectrometry
/
Monoamine Oxidase
Language:
En
Journal:
Chem Biodivers
Year:
2024
Type:
Article
Affiliation country:
Pakistan