[Cloning and functional analysis of AcFMO from onion during alliine biosynthesis].
Sheng Wu Gong Cheng Xue Bao
; 40(4): 1076-1088, 2024 Apr 25.
Article
in Zh
| MEDLINE
| ID: mdl-38658150
ABSTRACT
Flavin-containing monooxygenase (FMO) is the key enzyme in the biosynthesis pathway of CSOs with sulfur oxidation. In order to explore the molecular regulatory mechanism of FMO in the synthesis of onion CSOs, based on transcriptome database and phylogenetic analysis, one AcFMO gene that may be involved in alliin synthesis was obtained, the AcFMO had a cDNA of 1 374 bp and encoded 457 amino acids, which was evolutionarily closest to the AsFMO of garlic. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) indicated that AcFMO was the highest in the flowers and the lowest in the leaf sheaths. The results of subcellular localization showed that the AcFMO gene product was widely distributed throughout the cell A yeast expression vector was constructed, and the AcFMO gene was ecotopically overexpressed in yeast to further study the enzyme function in vitro and could catalyze the synthesis of alliin by S-allyl-l-cysteine. In summary, the cloning and functional identification of AcFMO have important reference value for understanding the biosynthesis of CSOs in onions.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Cloning, Molecular
/
Onions
/
Cysteine
Language:
Zh
Journal:
Sheng Wu Gong Cheng Xue Bao
Year:
2024
Type:
Article
Affiliation country:
China