Screening of active compounds with myocardial protective effects from Tongmai Yangxin pill / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 145-153, 2015.
Article
in Zh
| WPRIM
| ID: wpr-255219
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Based on cell model and HPLC-MS technology, to screen myocardial protection active compounds from traditional patent medicine Tongmai Yangxin pill (TMYXP).</p><p><b>METHODS</b>Fractions of TMYXP were prepared by high performance liquid preparation technology. The cardioprotective effects of prepared fractions were tested on H2O2 oxidation-damaged H9c2 myocardiocytes. The active components were analyzed by high performance liquid chromatography (HPLC) coupled with high resolution mass spectrometry. The possible active compounds were putatively identified by comparison of their MS ions and molecular weight with literatures.</p><p><b>RESULTS</b>Ten TMYXP components presented significant myocardial protective activities, 5 of which were investigated and presented good dose-effect relationships. Their median effective concentrations (EC50) were respectively 11.66, 17.44, 13.10, 7.332, 15.15 μg/mL. Totally, 11 potential active compounds were analyzed and identified, including Glycyrrhizic acid, Glycycoumarin, Licoisoflavone, Ophiopogonin D', Licoricon, Gancaonin L, Neoglycyrol, Emodin, Angeloylgomisin H, Angeloylgomisin Q and Glyasperin A.</p><p><b>CONCLUSION</b>The myocardial protection active compounds of TMYXP were screened successfully.</p>
Full text:
1
Database:
WPRIM
Traditional Medicines:
Medicinas_tradicionales_de_asia
/
Medicina_china
Main subject:
Mass Spectrometry
/
Drugs, Chinese Herbal
/
Cardiotonic Agents
/
Cell Line
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Myocytes, Cardiac
/
Hydrogen Peroxide
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2015
Type:
Article