Determination of five primary chemical constituents in Cassia angustifolia by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 363-365, 2008.
Article
in Zh
| WPRIM
| ID: wpr-324284
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.</p><p><b>METHOD</b>The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.</p><p><b>RESULT</b>The results showed that 5 effective components all separated well and showed good linearity.</p><p><b>CONCLUSION</b>The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.</p>
Full text:
1
Database:
WPRIM
Main subject:
Senna Extract
/
Temperature
/
Chemistry
/
Reproducibility of Results
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Chromatography, High Pressure Liquid
/
Anthraquinones
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Senna Plant
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Apigenin
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Glucosides
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Methods
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article