Fast and simultaneous determination of four index components in Qingwei Huanglian tablets by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2597-2600, 2007.
Article
in Zh
| WPRIM
| ID: wpr-324325
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a RP-HPLC method for fast and simultaneous determination of four index contents, which are Genpioside, Baicalin, Berberine Hydrochloride and Ammonium Glyeyrrhizinate, in Qingwei Huanglian tablets.</p><p><b>METHOD</b>A separation was well achieved by gradient elution on a Hypersil C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phases of acetontrile -0.5% triethylamine water-solution (pH 3.0) at a flow rate of 1.0 mL x min(-1), detection wavelength of 230 nm, and room temperature.</p><p><b>RESULT</b>The calibration curve were linear in the ranges of 4.82-77.2, 5.80-92.8, 1.63-26.1 and 6.40-102 microg x mL(-1) (r = 0.9997-0.9999) for genpioside, baicalin, berberine hydrochloride and ammonium glyeyrrhizinate, respectively. The average recoveries of four index components were not less than 98.0%.</p><p><b>CONCLUSION</b>The method is convenient, rapid and accurate. It is suitable for the quality control of Qingwei Huanglian tablets.</p>
Full text:
1
Database:
WPRIM
Traditional Medicines:
Medicinas_tradicionales_de_asia
/
Medicina_china
Therapeutic Methods and Therapies TCIM:
Terapias_biologicas
Main subject:
Plants, Medicinal
/
Quality Control
/
Tablets
/
Berberine
/
Flavonoids
/
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Glycyrrhizic Acid
/
Scutellaria baicalensis
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article