Effect of Siwu decoction on function and expression of P-glycoprotein in Caco-2 cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 933-937, 2015.
Article
in Zh
| WPRIM
| ID: wpr-330207
Responsible library:
WPRO
ABSTRACT
To study the effect of Siwu decoction on the function and expression of P-glycoprotein (P-gp) in Caco-2 cells. The Real-time quantitative poly-merase chain reaction (Q-PCR) was used to analyze the mRNA expression of MDR1 gene in Caco-2 cells. Flow cytometer was used to study the effect of Siwu decoction on the uptake of Rhodamine 123 in Caco-2 cells, in order to evaluate the efflux function of P-gp. Western blotting method was used to detect the effect of Siwu decoction on the P-gp protein expression of Caco-2 cells. Compared with the blank control group, after Caco-2 incubation with Siwu decoction at concentrations of 3.3, 5.0, 10.0 g x L(-1) for 24, 48, 72 h, the mRNA expression of MDR1 was up-regulated, suggesting the effect of Siwu decoction in inducing the expression of MDR1. After the administration with Siwu decoction in Caco-2 cells for 48 h, the uptake of Rhodamine 123 in Caco-2 cells decreased by respectively 16.6%, 22.1% (P < 0.05) and 45.4% (P < 0.01), indicating that the long-term administration of Siwu decoction can enhance the P-gp efflux function of Caco-2 cells. After the incubation of Caco-2 cells with Siwu decoction for 48 h, the P-gp protein expression on Caco-2 cell emebranes, demonstrating the effect of Siwu decoction in inducing the protein expression of P-gp.
Full text:
1
Database:
WPRIM
Traditional Medicines:
Medicinas_tradicionales_de_asia
/
Medicina_china
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Up-Regulation
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Caco-2 Cells
/
ATP Binding Cassette Transporter, Subfamily B
/
Genetics
/
Metabolism
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article