HPLC fingerprint of Huangkui Capsules and its application in quality control / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the HPLC fingerprint of traditional Chinese medicine Huangkui Capsules, thus to provide an approach and a basis for the quality control in production. METHODS: The analytical column was Thermo scientific Hypersil GOLD. The mobile phase was composed of acetonitrile (A) and 0.2% phosphoric acid (B) with gradient elution. The flow rate was 0.8 mL · min-1, and the detection wavelength was set at 360 nm with the column temperature maintained at 30°C. The fingerprints of the extracts from the raw materials, intermediate product, and Huangkui Capsules were compared. RESULTS: The similarities of the chromatograms of 10 different lots of Huangkui Capsules were over 0.95. The correlation was good between the raw materials, intermediate product and Huangkui capsules. CONCLUSION: This validated method is suitable for the quality evaluation and quality control of Huangkui Capsules.