GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
Journal of Veterinary Science
; : 241-248, 2013.
Article
in En
| WPRIM
| ID: wpr-92908
Responsible library:
WPRO
ABSTRACT
We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-beta-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Plant Extracts
/
Chromatography, High Pressure Liquid
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Coccidiostats
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Plant Roots
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Neospora
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Apiaceae
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Sophora
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Fruit
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Gas Chromatography-Mass Spectrometry
Language:
En
Journal:
Journal of Veterinary Science
Year:
2013
Type:
Article