A novel immunohistochemical detection system using mirror image complementary antibodies (MICA).
Histopathology
; 35(2): 129-33, 1999 Aug.
Article
en En
| MEDLINE
| ID: mdl-10460657
ABSTRACT
AIMS:
To describe and illustrate a novel and highly sensitive peroxidase-based immunohistochemical detection system which employs mutually attractive, mirror image complementary antibodies (MICA). METHODS ANDRESULTS:
To demonstrate the sensitivity of the MICA system alongside the avidin-biotin complex (ABC) method, we selected a range of mouse monoclonal and rabbit polyclonal primary antibodies against antigens that are generally regarded as relatively difficult or impossible to detect on formalin-fixed, paraffin-embedded lymphoid tissue. Compared with the ABC method, the MICA immunodetection method enabled us to dilute primary antibodies up to 200-fold with equivalent or superior immunostaining results and, usually, considerably shortened primary antibody incubation times.CONCLUSIONS:
We have described and illustrated a novel immunohistochemical detection system and demonstrated greatly increased sensitivity over the commonly used ABC system. An additional advantage of the MICA system is that it is avidin-free and so avoids non-specific staining due to endogenous tissue biotin.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Técnicas para Inmunoenzimas
/
Anticuerpos Monoclonales
/
Antígenos de Neoplasias
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Histopathology
Año:
1999
Tipo del documento:
Article
País de afiliación:
Reino Unido