Elucidating the biosynthesis of 2-carboxyarabinitol 1-phosphate through reduced expression of chloroplastic fructose 1,6-bisphosphate phosphatase and radiotracer studies with 14CO2.
Proc Natl Acad Sci U S A
; 99(7): 4742-7, 2002 Apr 02.
Article
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| MEDLINE
| ID: mdl-11917127
ABSTRACT
2-carboxyarabinitol 1-phosphate limits photosynthetic CO2 assimilation at low light because it is a potent, naturally occurring inhibitor of ribulose 1,5-bisphosphate carboxylase/oxygenase. Evidence is presented that this inhibitor is derived from chloroplastic fructose 1,6-bisphosphate. First, transgenic plants containing decreased amounts of chloroplastic fructose 1,6-bisphosphate phosphatase contained increased amounts of fructose 1,6-bisphosphate and 2-carboxyarabinitol 1-phosphate and greatly increased amounts of the putative intermediates hamamelose and 2-carboxyarabinitol, which in some cases were as abundant as sucrose. Second, French bean leaves in the light were shown to incorporate 14C from 14CO2 sequentially into fructose 1,6-bisphosphate, hamamelose bisphosphate, hamamelose monophosphate, hamamelose, and 2-carboxyarabinitol. As shown previously, 14C assimilated by photosynthesis was also incorporated into 2-carboxyarabinitol 1-phosphate during subsequent darkness.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Pentosafosfatos
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Solanum tuberosum
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Cloroplastos
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Fructosa-Bifosfatasa
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2002
Tipo del documento:
Article
País de afiliación:
Reino Unido