Chemokine receptor CXCR2 regulates the functional properties of AMPA-type glutamate receptor GluR1 in HEK cells.
J Neuroimmunol
; 129(1-2): 66-73, 2002 Aug.
Article
en En
| MEDLINE
| ID: mdl-12161022
ABSTRACT
Experiments were conducted in both HEK cells and cerebellar neurons to investigate whether CXC chemokine receptor 2 (CXCR2) is functionally coupled to GluR1. The co-expression of CXCR2 with GluR1 in HEK cells increased (i) the GluR1 "apparent" affinity for the transmitter; (ii) the GluR1 channel open probability; and (iii) GluR1 binding site cooperativity upon CXCR2 stimulation with CXC chemokine ligand 2 (CXCL2). The affinity of C-terminal-deleted GluR1 for glutamate (Glu) remained stable instead. Furthermore, CXCL2 increased the binding site cooperativity of AMPA receptors in rat cerebellar granule cells; and the amplitude of spontaneous excitatory postsynaptic current (sEPSCs) in Purkinje neurons (PNs). Our findings indicate that the coupling of CXCR2 with GluR1 may modulate glutamatergic synaptic transmission.
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Bases de datos:
MEDLINE
Asunto principal:
Sinapsis
/
Sistema Nervioso Central
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Receptores AMPA
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Transmisión Sináptica
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Ácido Glutámico
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Quimiocinas CXC
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Receptores de Interleucina-8B
Idioma:
En
Revista:
J Neuroimmunol
Año:
2002
Tipo del documento:
Article
País de afiliación:
Italia