[Orexin A and neuropeptide Y levels in plasma and hypothalamus of rats with chronic renal failure].
Zhonghua Yi Xue Za Zhi
; 83(11): 992-5, 2003 Jun 10.
Article
en Zh
| MEDLINE
| ID: mdl-12899803
ABSTRACT
OBJECTIVE:
To explore the changes of orexin A and neuropeptide (NPY) in plasma and hypothalamus of rats with chronic renal failure (CRF).METHODS:
41 male Wister rats weighing 200 approximately 250 g were randomly divided into three groups normal group, sham operation group, and CRF group (with the right kidney and 2/3 of the left kidney resected). A certain number of rats were decapitated 4, 8,and 12 weeks after respectively. Their hypothalami were removed and blood collected. Radioimmunoassay was used to measure the levels of orexin A and NPY in hypothalamus and plasma. Automatic biochemical analyzer was used to measure the serum creatinine.RESULTS:
The serum creatinine level of CRF rats was both significantly higher than those of the sham operation rats at week 8 and week 12, respectively. The plasma orexin A level of CRF rats at week 12 was 264 pg/ml +/- 62 pg/ml, significantly higher than that of sham operation group (183 pg/ml +/- 56pg/ml, P = 0.039). The hypothalamus orexin A level of CRF rats were 10.5 fmol/mg +/- 2.7 fmol/mg wet weight at week 12, significantly lower than that of sham operation rats (17.4 fmol/mg +/- 3.9 fmol/mg wet weight, P = 0.023). The plasma NPY levels of CRF rats at week 8 and week 12 were significantly higher than those of the sham operation rats (7.1 pmol/ml +/- 1.7 pmol/ml vs 5.0 pmol/ml +/- 0.5 pmol/ml, P = 0.01; and 7.9 pmol/ml +/- 1.1 pmol/ml vs 4.8 pmol/ml +/- 1.1 pmol/ml, P = 0.0008). The hypothalamus NPY level of CRF rats at week 12 were 70 fmol/mg +/- 23 fmol/mg wet weight, significantly lower than that of the sham operation rats (113 fmol/mg +/- 31 fmol/mg wet weight, P = 0.03).CONCLUSION:
Loss of renal function may diminish the excretion of orexin A and neuropeptide. The lowering of hypothalamus orexin A and neuropeptide Y levels may be one of the causes inducing anorexia in CRF.
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Bases de datos:
MEDLINE
Asunto principal:
Neuropéptido Y
/
Neuropéptidos
/
Proteínas Portadoras
/
Péptidos y Proteínas de Señalización Intracelular
/
Hipotálamo
/
Fallo Renal Crónico
Idioma:
Zh
Revista:
Zhonghua Yi Xue Za Zhi
Año:
2003
Tipo del documento:
Article
País de afiliación:
China