Plasma membrane Ca2+ ATPase 4 is required for sperm motility and male fertility.
J Biol Chem
; 279(27): 28220-6, 2004 Jul 02.
Article
en En
| MEDLINE
| ID: mdl-15078889
ABSTRACT
Calcium and Ca(2+)-dependent signals play a crucial role in sperm motility and mammalian fertilization, but the molecules and mechanisms underlying these Ca(2+)-dependent pathways are incompletely understood. Here we show that homozygous male mice with a targeted gene deletion of isoform 4 of the plasma membrane calcium/calmodulin-dependent calcium ATPase (PMCA), which is highly enriched in the sperm tail, are infertile due to severely impaired sperm motility. Furthermore, the PMCA inhibitor 5-(and-6)-carboxyeosin diacetate succinimidyl ester reduced sperm motility in wild-type animals, thus mimicking the effects of PMCA4 deficiency on sperm motility and supporting the hypothesis of a pivotal role of the PMCA4 on the regulation of sperm function and intracellular Ca(2+) levels.
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Bases de datos:
MEDLINE
Asunto principal:
Motilidad Espermática
/
ATPasas Transportadoras de Calcio
/
Fertilidad
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Biol Chem
Año:
2004
Tipo del documento:
Article
País de afiliación:
Alemania