[Serapharmacological effect of kangxian ruangan granula on the proliferation of hepatic stellate cells].

ABSTRACT

OBJECTIVE: To investigate the antifibrotic mechanism of kangxian ruangan granula (KXRGG) at cellular level. METHOD: HSC were isolated from the normal rats by enzyme digestion and density gradient centrifugation, cultured, identified and subcultured. The medicated sera were prepared to incubated with the subcultured HSC. Sodium chloride sera and colchicines sera were taken as control groups. HSC viability was observed by 3H-proline; cell proliferation was measured with 3H-TdR incorporation and MTT colorimetric assay. RESULT: KXRGG medicated serum had no effect on the cellular shape and elevated 3H-proline incorporation (P < 0.01). Drug serum obviously inhibited 3H-TdR incorporation and MTT transformation (P < 0.01), and the latter had a drug concentration-dependent tendency. CONCLUSIONS: KXRGG can elevate cellular viability and has no toxic effect. The formula can inhibit the proliferation of HSC, which may be one of the cellular mechanisms of its antifibrotic action.