[Determination of berberine in human serum by reversed-phase high performance liquid chromatography].
Se Pu
; 15(5): 454-5, 1997 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-15739507
ABSTRACT
Berberine is a safe and effective anti-arrhythmic drug. We do not clearly know about the dose-effect relationship. Besides, berberine is not fit for administration by i.m. and i.v.. Therefore, it is necessary to build a PD model of berberine, study its pharmacodynamics parameters in vivo, and provide a concrete criteria in the clinical usage. In this article, we set up a method for determination of concentration of berberine in human serum by RP-HPLC with Lichrosorb RP-18 column (250 mm x 4 mm) and mobile phase of MeOH containing 0.15% triethylamine. The content of berberine was determined by the external standard method with UV detector at 347 nm. The linear range was 0.2-2 mg/L (r = 0.9996). The detectable limit was 2.55 ng. The average recovery was 90.73% and the coefficients of variation were below 8.0%. The method is sensitive and accurate.
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Bases de datos:
MEDLINE
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Asunto principal:
Berberina
/
Medicamentos Herbarios Chinos
/
Cromatografía Líquida de Alta Presión
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Se Pu
Año:
1997
Tipo del documento:
Article