[Effects of selenium on benzo[a] pyrene-induce DNA damage in mouse lung cells].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 22(6): 445-7, 2004 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-15748481
ABSTRACT
OBJECTIVE:
To explore the effects of selenium on DNA damage induced by benzo[a] pyrene (BaP) in mouse lung cells.METHODS:
Sodium selenite was given to Kunming male mice by i.p. and BaP was given by oral gavage. The control group was given solvent only with the same method. DNA damage was detected by single cell gel electrophoresis (or comet assay).RESULTS:
The damage degrees in mice treated with 125, 250 and 500 mg/kg of BaP were more severe than that of control (P < 0.01). The rates of comet cells in the BaP-treated groups (43.50%, 84.00%, 95.63%) were significantly higher than that of control (9.75%, P < 0.01), and there was obvious dose-response relationship. 0.75, 1.50 and 3.00 mg/kg of sodium selenite presented antagonistic effects against DNA damage induced by 250 mg/kg of BaP in mouse's lung cells. The antagonistic effect of sodium selenite at the dose of 1.50 mg/kg was better than those of sodium selenite at the doses of 0.75, 3.00 mg/kg.CONCLUSION:
BaP at the doses of 125 approximately 500 mg/kg could significantly induce DNA damage of lung cells in mice. 0.75 approximately 3.00 mg/kg of sodium selenite could inhibit DNA damage of lung cells in mice induced by 250 mg/kg of BaP.
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Bases de datos:
MEDLINE
Medicinas Complementárias:
Homeopatia
Asunto principal:
Selenio
/
Benzo(a)pireno
/
Daño del ADN
Idioma:
Zh
Revista:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Año:
2004
Tipo del documento:
Article
País de afiliación:
China