Molecular and biomechanical characterization of mineralized tissue by dental pulp cells on titanium.
J Dent Res
; 84(6): 515-20, 2005 Jun.
Article
en En
| MEDLINE
| ID: mdl-15914587
ABSTRACT
The application of implant therapy is still limited, because of various risk factors and the long healing time required for bone-titanium integration. This study explores the potential for osseointegration engineering with dental pulp cells (DPCs) by testing a hypothesis that DPCs generate mineralized tissue on titanium. DPCs extracted from rat incisors positive for CD44, alkaline phosphatase activity, and mineralizing capability were cultured on polystyrene and on machined and dual-acid-etched (DAE) titanium. Tissue cultured on titanium with a Ca/P ratio of 1.4 exhibited plate-like morphology, while that on the polystyrene exhibited fibrous and punctate structures. Tissues cultured on titanium were harder than those on polystyrene, 1.5 times on the machined and 3 times on the DAE. Collagen I, osteopontin, and osteocalcin genes were up-regulated on titanium, especially the DAE surface. In conclusion, DPCs showing some characteristics of the previously identified dental pulp stem cells can generate mineralized tissue on titanium via the osteoblastic phenotype, which can be enhanced by titanium surface roughness.
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Bases de datos:
MEDLINE
Asunto principal:
Titanio
/
Calcificación Fisiológica
/
Pulpa Dental
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
J Dent Res
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos