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[The effects of triptolide inhibition of mouse lymphocytes activated in vitro].
Yu, Yu; Zeng, Yaoying; Liu, Liang; Ji, Yuhua; Zhao, Jinxian.
Afiliación
  • Yu Y; Key Laboratory of Tissue Transplantation & Immunology of Ministy of Educational Specialized Laboratory, Jinan University, Guangzhou.
Zhong Yao Cai ; 28(6): 499-502, 2005 Jun.
Article en Zh | MEDLINE | ID: mdl-16209269
OBJECTIVE: To study the effects of triptolidel on murine T lymphocytes activated. METHODS: We isolated and cultured the mouse lymphocytes from lymph nodes of mice in vitro. The lymphocytes were pre-treated triptolide for 1 h prior to activation with ConA. After 6 hours stimulation, determined IL-2 and IFN mRNA expression by RT-PCR. CD69 expression of mouse CD3+ T cell was determined by multi-color flow cytometric analysis after 6 hours stimulation, and CD25 expression of mouse CD3+ T cell was determined by multi-color flow cytometric analysis after 24 hours stimulation. RESULTS: TRD could inhibit IL-2 and IFN-gamma mRNA expression. It also inhibited CD69 and CD25 expression of mouse CD3+ T cell. CONCLUSION: the immunosuppressive mechanism of TRD may be related to the inhibitory effects on IL-2 and IFN-gamma mRNA expression and the inhibition of CD69 and CD25 expression.
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Bases de datos: MEDLINE Medicinas Tradicionales: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas Asunto principal: Fenantrenos / Plantas Medicinales / Activación de Linfocitos / Diterpenos Idioma: Zh Revista: Zhong Yao Cai Año: 2005 Tipo del documento: Article
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Bases de datos: MEDLINE Medicinas Tradicionales: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas Asunto principal: Fenantrenos / Plantas Medicinales / Activación de Linfocitos / Diterpenos Idioma: Zh Revista: Zhong Yao Cai Año: 2005 Tipo del documento: Article