[The study on the extraction and the antivirus activity of amylose extracted from Grateloupia filicina].

ABSTRACT

OBJECTIVE: To determine the activity of anti-Herpes simplex II virus (HSV-2) of amylose extracted from Grateloupia filicina. METHODS: Grateloupia filicina amylose was extracted by five kinds of abstraction methods and its suppression on Herpes simplex II virus was observed on cell level in three aspects the drug activity of protecting cell, the drug influence of HSV-2 proliferation and the drug synthesis action of HSV-2. RESULTS: Grateloupia filicina had prominence anti-Herpes simplex II virus activity, IC50 of amylose extracted by water distilling and ethanol sedimentation was 5.80 microg/ml. CONCLUSION: It suggest that the antivirus activity happen in the stage of HSV-2 binding, adsorption and ingression with Vero cell.