Recombinant expression of Garlic virus C (GARV-C) capsid protein in insect cells and its potential for the production of specific antibodies.
Microbiol Res
; 163(3): 354-61, 2008.
Article
en En
| MEDLINE
| ID: mdl-16890415
ABSTRACT
Garlic cultivars in Brazil are infected by a complex of viruses and for some virus species, such as the allexivirus, purification of the virions is sometimes cumbersume. To overcome this problem, recombinant expression of viral proteins in heterologous systems is an alternative method for producing antibodies. The capsid gene from Garlic virus C (GarV-C), an Allexivirus, was inserted into the genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) generating the recombinant virus vSynGarV-C. The recombinant protein expression was confirmed by SDS-PAGE and western-blot of extracts from recombinant virus infected insect cells, where a protein band of approximately 32KDa was observed only in extracts from recombinant infected cells. This protein corresponded to the predicted size of the capsid protein of the GarV-C. A rabbit polyclonal antibody was raised against this protein, shown to be specific for the GarV-C protein in western-blot and dot-Elisa, however with a low titer.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Proteínas de la Cápside
/
Flexiviridae
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Microbiol Res
Año:
2008
Tipo del documento:
Article
País de afiliación:
Brasil