[The effect of icariin and astragalosid I on the proliferation and differentiation of bone marrow stromal cells].

ABSTRACT

OBJECTIVE: To observe the effects of icariin and astragalosid I on the proliferative and alkaline phosphatase (ALP) activity of dog bone marrow stromal cells (BMSCs). METHODS: The dog's BMSCs were isolated and cultured in vitro. The 3th generation BMSCs were treated with icariin or astragalosid I at the concentration of 50 ng/ml and compared with BMSCs of BMP-2 group and control group. The growth curves of BMSCs were drawn by 3-(4,5-dimiethylthiazole-2-yl)-2, 5-hiphenyl tetrazolium bromide (MTT) colorimetric assay every day from the 1st to the 8th day to estimate the proliferative ability of BMSCs. The curves of OD value of ALP excreted by BMSCs on the 1st, 3th, 6th, 10th and the 14th day were recorded to estimate the ALP activity of BMSCs. RESULTS: After the pertreatment with icariin and astragalosid I, the BMSCs acquired higher MTF values and higher ALP's OD values as compared with control group and the difference between experiment group and control group was statistically significant (P < 0.05). CONCLUSION: Icariin and Astragealosid I can accelerate the proliferation and ALP excretion of BMSCs. At the same time, the osteogenesis ability of these cells is greatly improved.