Inner anti-inflammatory mechanisms of petroleum ether extract from Melilotus suaveolens Ledeb.
Inflammation
; 30(6): 213-23, 2007 Dec.
Article
en En
| MEDLINE
| ID: mdl-17763929
ABSTRACT
UNLABELLED Melilotus suaveolens Ledeb is a species of traditional medical plant for treating inflammation-related disease. This study is to explore the inner anti-inflammatory mechanism on petroleum ether extract from Melilotus suaveolens Ledeb. MATERIALS AND METHODS:
Inflammatory cellular model was founded by intervention of lipopolysaccharide (LPS) on RAW264.7 cell line. Secretion of TNF-alpha, IL-1beta, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-alpha, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, activation of NF-kappaB and ingredients in the extract were assayed.RESULTS:
The extract could not only reduce production of pro-inflammatory mediators by blocking NF-kappaB activation but promote release of anti-inflammatory mediator HO-1 significantly. The only active ingredient in the extract was coumarin and the concentration of coumarin in each 1 g extract was 0.27822 mg.CONCLUSION:
Compared to Dexamethasone, the extract not only has similar effects on antagonizing pro-inflammatory mediators and cytokines but has effects on promoting production of anti-inflammatory mediators.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Solventes
/
Extractos Vegetales
/
Melilotus
/
Alcanos
/
Macrófagos
/
Antiinflamatorios
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Inflammation
Año:
2007
Tipo del documento:
Article