Comparison of rapid DNA extraction methods applied to PCR identification of medicinal mushroom Ganoderma spp.
Prep Biochem Biotechnol
; 37(4): 369-80, 2007.
Article
en En
| MEDLINE
| ID: mdl-17849291
ABSTRACT
Four different DNA extraction methods were used to extract genomic DNA of the medicinal mushroom Lingzhi from its developing stage materials, such as mycelium, dry fruiting body, or sliced and spore powder or sporoderm-broken spore powder. The DNA samples were analyzed using agarose gel electrophoresis, UV spectrophotometer, and PCR amplification. According to the average yields and purity of DNA, high salt concentrations and low pH methods were the best for DNA extraction. The mycelia and sporoderm-broken spore powder yielded higher and purer DNA. The method developed could effectively eliminate the influence of the secondary metabolites to DNA extraction. The DNA samples extracted from the developed method could be successfully used for PCR applications.
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Bases de datos:
MEDLINE
Métodos Terapéuticos y Terapias MTCI:
Plantas_medicinales
Asunto principal:
Biotecnología
/
ADN
/
Reacción en Cadena de la Polimerasa
/
Ganoderma
/
Medicina Tradicional de Asia Oriental
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Prep Biochem Biotechnol
Año:
2007
Tipo del documento:
Article