Extraction of proteins from switchgrass using aqueous ammonia within an integrated biorefinery.
Appl Biochem Biotechnol
; 143(2): 187-98, 2007 Nov.
Article
en En
| MEDLINE
| ID: mdl-18025607
ABSTRACT
Switchgrass (Panicum vergatum) is a potential feedstock for future cellulosic biorefineries. Such a feedstock may also provide protein, most likely for use as an animal feed. In this paper, we present a potential scheme for integrating fiber processing with extractions to obtain both sugar and protein products from switchgrass pretreated using Ammonia Fiber Expansion (AFEX). Solutions of 3% aqueous ammonia at pH 10.5 provided optimal extraction of proteins. Addition of the nonionic surfactant Tween-80 improved protein recovery for AFEX-treated materials. It was determined that an extraction following AFEX solubilized approximately 40% of the protein, while a subsequent hydrolysis solubilized much of the remaining protein while producing 325 g sugar per kg biomass. The remaining insoluble residue contained very little protein or ash, making it ideal for heat and power production. In contrast, an extraction following hydrolysis solubilized only 68% of the original protein in the biomass, while obtaining slightly higher sugar yields.
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Bases de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Extractos Vegetales
/
Amoníaco
/
Panicum
Idioma:
En
Revista:
Appl Biochem Biotechnol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos