EPR studies on the superoxide-scavenging capacity of the nutraceutical resveratrol.
Mol Cell Biochem
; 313(1-2): 187-94, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-18409032
ABSTRACT
Resveratrol (3,4',5-trihydroxystilbene), a polyphenolic compound found in mulberries, grapes, and red wine, has received considerable attention because of its apparent protective effects against various degenerative diseases due to its potential antioxidant activities. However, direct evidence for the superoxide-scavenging capacity of resveratrol is lacking in literature. In this study, electron paramagnetic resonance spectroscopy in combination with 5-(diethoxyphosphoryl)-5-methylpyrroline-N-oxide (DEPMPO)-spin trapping technique was utilized to determine the ability of resveratrol in scavenging superoxide anions generated from both potassium superoxide and the xanthine oxidase/xanthine system. We have demonstrated here for the first time that the presence of resveratrol resulted in decreased formation of DEPMPO-superoxide adduct (DEPMPO-OOH) in both the potassium superoxide and xanthine oxidase/xanthine systems, indicating that resveratrol could directly scavenge superoxide anions. The inhibition of DEPMPO-OOH in the xanthine oxidase/xanthine system, however, was found to be much potent as compared to that observed in potassium superoxide system. It was further shown that resveratrol could also directly inhibit xanthine oxidase activity as assessed by oxygen consumption and formation of uric acid. Taken together, the dual role of resveratrol in directly scavenging superoxide and inhibiting its generation via xanthine oxidase reported in this study may explain, at least in part, the protective role of this compound against oxidative injury in various disease processes.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Estilbenos
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Depuradores de Radicales Libres
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Superóxidos
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Suplementos Dietéticos
Idioma:
En
Revista:
Mol Cell Biochem
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos