Purified extract from Clematis mandshurica prevents adenoviral-TRAIL induced apoptosis on rat articular chondrocytes.
Am J Chin Med
; 36(2): 399-410, 2008.
Article
en En
| MEDLINE
| ID: mdl-18457369
ABSTRACT
Since TNF-related apoptosis inducing ligand (TRAIL) is one of several apoptotic stimuli on articular chondrocytes, the modulation of the mechanism mediated by TRAIL could be considered as a novel strategy for the treatment of osteoarthritis (OA). Previous studies demonstrated that Clematis mandshurica prevents staurosporin-induced apoptosis in articular chondrocytes. This study was undertaken to examine whether Clematis mandshurica could prevent TRAIL-induced apoptosis in articular chondrocytes. Our data show that Clematis mandshurica prevents adenoviral TRAIL (Ad-TRAIL)-induced apoptosis in primary cultured articular chondrocytes. Clematis mandshurica prevents Ad-TRAIL-induced down-regulation of 14-3-3 and phosphorylated Akt. In addition, Clematis mandshurica treatment prevents the Ad-TRAIL-induced reduction of the interactions between 14-3-3 with phospho-ser112-Bad and phospho-ser136-Bad, and BcL-xL with phospho-ser155-Bad. A better understanding of the mechanism underlying inhibition of apoptosis in OA chondrocytes by Clematis mandshurica might lead to the development of a new therapeutic strategy for OA.
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Bases de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Apoptosis
/
Condrocitos
/
Clematis
/
Ligando Inductor de Apoptosis Relacionado con TNF
/
Articulaciones
Tipo de estudio:
Etiology_studies
Idioma:
En
Revista:
Am J Chin Med
Año:
2008
Tipo del documento:
Article
País de afiliación:
Corea del Sur