Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposome chromatography column and reversed-phase column for separation of complex traditional Chinese medicine Longdan Xiegan Decoction.
J Chromatogr A
; 1216(11): 2185-91, 2009 Mar 13.
Article
en En
| MEDLINE
| ID: mdl-18585729
ABSTRACT
A comprehensive two-dimensional HPLC system with an immobilized liposome chromatography (ILC) column in conjunction with an RP column (in tandem) was developed for the screening and analysis of the membrane-permeable compounds in a traditional Chinese medicine prescription Longdan Xiegan Decoction (LXD). More than 50 components in LXD were resolved using the developed separation system. Eight flavonoids and two iridoids were identified interacting with the ILC column; a system that mimics biomembranes. The results show that the developed comprehensive two-dimensional chromatography system can be used for identifying membrane permeable natural products in complex matrixes such as extracts of traditional Chinese medicine prescriptions.
Texto completo:
1
Bases de datos:
MEDLINE
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Asunto principal:
Flavonoides
/
Medicamentos Herbarios Chinos
/
Cromatografía Líquida de Alta Presión
/
Iridoides
Idioma:
En
Revista:
J Chromatogr A
Año:
2009
Tipo del documento:
Article
País de afiliación:
China