CGR3: a Golgi-localized protein influencing homogalacturonan methylesterification.
Mol Plant
; 4(5): 832-44, 2011 Sep.
Article
en En
| MEDLINE
| ID: mdl-21422118
ABSTRACT
Plant cell walls are complex structures that offer structural and mechanical support to plant cells and are ultimately responsible for plant architecture and form. Pectins are a large group of complex polysaccharides of the plant cell wall that are made in the Golgi and secreted to the wall. The methylesterification of pectins is believed to be an important factor for the dynamic properties of the cell wall. Here, we report on a protein of unknown function discovered using an extensive proteomics analysis of cotton Golgi. Through bioinformatic analyses, we identified the ortholog of such protein, here named cotton Golgi-related 3 (CGR3) in Arabidopsis and found that it shares conserved residues with S-adenosylmethionine methyltransferases. We established that CGR3 is localized at the Golgi apparatus and that the expression of the CGR3 gene is correlated with that of several cell wall biosynthetic genes, suggesting a possible role of the protein in pectin modifications. Consistent with this hypothesis, immunofluorescence microscopy with antibodies for homogalacturonan pectins (HG) indicated that the cell walls of cgr3 knockout mutants and plants overexpressing CGR3 are decreased and increased in HG methylesterification, respectively. Our results suggest that CGR3 plays a role in the methylesterification of homogalacturonan in Arabidopsis.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Pectinas
/
Arabidopsis
/
Proteínas de Arabidopsis
/
Aparato de Golgi
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Mol Plant
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos