Isolation of transcripts overexpressed in the human pathogen Trichophyton rubrum grown in lipid as carbon source.
Can J Microbiol
; 57(4): 333-8, 2011 Apr.
Article
en En
| MEDLINE
| ID: mdl-21491985
ABSTRACT
Trichophyton rubrum is the most common etiological agent of human dermatophytosis. Despite the incidence and medical importance of this dermatophyte, little is known about the mechanisms of host invasion and pathogenicity. Host invasion depends on the adaptive cellular responses of the pathogen that allow it to penetrate the skin layers, which are mainly composed of proteins and lipids. In this study, we used suppression subtractive hybridization to identify transcripts overexpressed in T. rubrum cultured in lipid as carbon source. Among the subtractive cDNA clones isolated, 85 clones were positively screened by cDNA array dot blotting and were sequenced. The putative proteins encoded by the isolated transcripts showed similarities to fungal proteins involved in metabolism, signaling, defense, and virulence, such as the MDR/ABC transporter, glucan 1,3-ß-glucosidase, chitin synthase B, copper-sulfate-regulated protein, and serine/threonine phosphatase (calcineurin A). These results provide the first molecular insight into the genes differentially expressed during the adaptation of T. rubrum to a lipidic carbon source.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Trichophyton
/
Aceites de Plantas
/
Regulación Fúngica de la Expresión Génica
Idioma:
En
Revista:
Can J Microbiol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Brasil