Development of a MR-visible compound for tracing neuroanatomical connections in vivo.
Neuron
; 70(2): 229-43, 2011 Apr 28.
Article
en En
| MEDLINE
| ID: mdl-21521610
ABSTRACT
Traditional studies of neuroanatomical connections require injection of tracer compounds into living brains, then histology of the postmortem tissue. Here, we describe and validate a compound that reveals neuronal connections in vivo, using MRI. The classic anatomical tracer CTB (cholera-toxin subunit-B) was conjugated with a gadolinium-chelate to form GdDOTA-CTB. GdDOTA-CTB was injected into the primary somatosensory cortex (S1) or the olfactory pathway of rats. High-resolution MR images were collected at a range of time points at 11.7T and 7T. The transported GdDOTA-CTB was visible for at least 1 month post-injection, clearing within 2 months. Control injections of non-conjugated GdDOTA into S1 were not transported and cleared within 1-2 days. Control injections of Gd-Albumin were not transported either, clearing within 7 days. These MR results were verified by classic immunohistochemical staining for CTB, in the same animals. The GdDOTA-CTB neuronal transport was target specific, monosynaptic, stable for several weeks, and reproducible.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Vías Olfatorias
/
Corteza Somatosensorial
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Imagen por Resonancia Magnética
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Neuroanatomía
Idioma:
En
Revista:
Neuron
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos