A fast method coupling ultrahigh performance liquid chromatography with diode array detection for flavonoid quantification in citrus fruit extracts.
J Agric Food Chem
; 59(12): 6353-9, 2011 Jun 22.
Article
en En
| MEDLINE
| ID: mdl-21591716
ABSTRACT
Flavonoids are a widely distributed group of polyphenolic compounds present in an extensive range of edible plants, notably Citrus species. This article reports a rapid, optimized, and validated method for the separation and quantification of flavonoids in three Citrus fruit extracts by ultrahigh performance liquid chromatography (UHPLC) using a photodiode array detector. This new procedure allowed the simultaneous separation and quantification of 11 selected flavonoids in 5.5 min, 8.2 times faster than that by HPLC analysis. The solvent consumption for each individual analysis was also reduced almost 6.2-fold. The most abundant component in the analyzed samples was naringin (299.06-544.36 mg 100 g⻹), followed by rutin (116.60-256.33 mg 100 g⻹) and quercetin (7.78-251.49 mg 100 g⻹). Isoquercitrin was found in a lower proportion (60.05-81.88 mg 100 g⻹). The method was completely validated, providing a sensitive analysis for flavonoid detection and showing satisfactory data for all the parameters tested. This methodology is cheaper, more environmentally friendly, and easier to perform than others previously described.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Flavonoides
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Extractos Vegetales
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Cromatografía Líquida de Alta Presión
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Citrus
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
J Agric Food Chem
Año:
2011
Tipo del documento:
Article
País de afiliación:
España