Effect of saucerneol D on melanin production in cAMP-elevated melanocytes.
Arch Pharm Res
; 34(8): 1339-45, 2011 Aug.
Article
en En
| MEDLINE
| ID: mdl-21910056
ABSTRACT
Intracellular cAMP stimulates microphthalmia-associated transcription factor (MITF) induction in melanocytes through cAMP-responsive element binding protein (CREB), which plays a pivotal role in the gene expression of tyrosinase for melanin biosynthesis. In the present study, saucerneol D as a lignan constituent of Saururus chinensis (Saururaceae family) efficiently inhibited melanin production with IC(50) values of 188-297 nM in B16 melanoma cells stimulated with α-melanocyte stimulating hormone (α-MSH) or other cAMP elevators. Moreover, saucerneol D down-regulated α-MSH-induced gene expression of tyrosinase at the transcription level in B16 cells, but it did not directly inhibit the catalytic activity of cell-free tyrosinase. As to the molecular basis of hypopigmenting action, saucerneol D inhibited α-MSH-induced phosphorylation of CREB in the cells, and sequentially suppressed MITF induction. Taken together, this study provides saucerneol D down-regulated the gene expression of tyrosinase, resulting in the inhibition of cAMP-induced melanin biosynthesis, and suggests pharmacological potential of the lignan structure in skin hyperpigmentation.
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1
Bases de datos:
MEDLINE
Asunto principal:
Lignanos
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AMP Cíclico
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Melaninas
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Melanocitos
Idioma:
En
Revista:
Arch Pharm Res
Año:
2011
Tipo del documento:
Article