Calreticulin as a hydrophilic chimeric molecular adjuvant enhances IgG responses to the spike protein of severe acute respiratory syndrome coronavirus.
Microbiol Immunol
; 56(8): 554-61, 2012 Aug.
Article
en En
| MEDLINE
| ID: mdl-22530918
ABSTRACT
Fragment 450-650 of the spike (S) protein (S450-650) of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) contains epitopes capable of being recognized by convalescent sera of SARS patients. Vaccination of mice with recombinant S450-650 (rS450-650) can induce Abs against SARS-CoV, although the titer is relatively low. In the present study, a fusion protein linking a fragment (residues 39-272) of murine calreticulin (CRT) to S450-650 in a prokaryotic expression system was created. Compared with target antigen alone, the recombinant fusion product (rS450-650-CRT) has much improved hydrophilicity and immunogenicity. The S450-650-specific IgG Abs of BALB/c mice subcutaneously immunized with rS450-650-CRT were in substantially higher titer (approximately fivefold more). Furthermore, the fusion protein, but not rS450-650 alone, was able to elicit S450-650-specific IgG responses in T cell deficient nude mice. Given that rCRT/39-272 can drive the maturation of bone-marrow-derived dendritic cells, directly activate macrophages and B cells, and also elicit helper T cell responses in vivo, we propose that fragment 39-272 of CRT is an effective molecular adjuvant capable of enhancing target Ag-specific humoral responses in both a T cell-dependent and independent manner. Fusion protein rS450-650-CRT is a potential candidate vaccine against SARS-CoV infection.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Inmunoglobulina G
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Glicoproteínas de Membrana
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Vacunas Virales
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Proteínas del Envoltorio Viral
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Adyuvantes Inmunológicos
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Calreticulina
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Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
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Anticuerpos Antivirales
Idioma:
En
Revista:
Microbiol Immunol
Año:
2012
Tipo del documento:
Article
País de afiliación:
China