A method for improved resolution for fluorescence microscopy using plastic-embedded material subjected to resin extraction.
Stain Technol
; 65(5): 259-61, 1990.
Article
en En
| MEDLINE
| ID: mdl-2278052
ABSTRACT
A protocol is given that uses NaOH, benzene, acetone and methanol to extract epoxy resins from semithin sections. Such sections appear superior to paraffin or unsectioned materials for fluorescence microscopic observations. Use of ultrarapid films (e.g., Kodak T-Max P3200) at ISO 3200 minimizes fading without use of antifading agents and without introducing unacceptable photographic grain size.
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Bases de datos:
MEDLINE
Asunto principal:
Plantas
/
Técnicas Histológicas
/
Resinas Epoxi
/
Microscopía Fluorescente
Idioma:
En
Revista:
Stain Technol
Año:
1990
Tipo del documento:
Article