Saponins from Platycodon grandiflorum inhibit hepatic lipogenesis through induction of SIRT1 and activation of AMP-activated protein kinase in high-glucose-induced HepG2 cells.
Food Chem
; 140(1-2): 115-23, 2013 Sep 01.
Article
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| MEDLINE
| ID: mdl-23578622
ABSTRACT
Saponins from the roots of Platycodon grandiflorum (Changkil saponins, CKS) have antioxidant and hepatoprotective properties. This study investigated the effects of CKS on AMP-activated protein kinase (AMPK) activation and hepatic lipogenesis in HepG2 cells. CKS suppressed high-glucose-induced lipid accumulation and inhibited high-glucose-induced fatty acid synthase (FAS) and sterol regulatory element binding protein-1c (SREBP-1c) expression in HepG2 cells. Moreover, the use of a pharmacological AMPK inhibitor revealed that AMPK is essential for the suppression of SREBP-1c expression in CKS-treated cells. Finally, the activation of calcium/calmodulin-dependent kinase kinase ß (CaMKKß) and SIRT1 was necessary for CKS-enhanced activation of AMPK. These results indicate that CKS prevents lipid accumulation in HepG2 cells by blocking the expression of SREBP-1c and FAS through SIRT1 and CaMKKß/AMPK activation. Using CKS to target AMPK activation may provide a promising approach for the prevention lipogenesis.
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Bases de datos:
MEDLINE
Asunto principal:
Saponinas
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Extractos Vegetales
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Regulación hacia Abajo
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Platycodon
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Lipogénesis
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Proteínas Quinasas Activadas por AMP
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Sirtuina 1
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Glucosa
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En
Revista:
Food Chem
Año:
2013
Tipo del documento:
Article