Fiberoptic microneedle device facilitates volumetric infusate dispersion during convection-enhanced delivery in the brain.
Lasers Surg Med
; 45(7): 418-26, 2013 Sep.
Article
en En
| MEDLINE
| ID: mdl-23861185
ABSTRACT
BACKGROUND AND OBJECTIVES:
A fiberoptic microneedle device (FMD) was designed and fabricated for the purpose of enhancing the volumetric dispersal of macromolecules delivered to the brain through convection-enhanced delivery (CED) by concurrent delivery of sub-lethal photothermal hyperthermia. This study's objective was to demonstrate enhanced dispersal of fluid tracer molecules through co-delivery of 1,064 nm laser energy in an in vivo rodent model. MATERIALS ANDMETHODS:
FMDs capable of co-delivering fluids and laser energy through a single light-guiding capillary tube were fabricated. FMDs were stereotactically inserted symmetrically into both cerebral hemispheres of 16 anesthetized rats to a depth of 1.5 mm. Laser irradiation (1,064 nm) at 0 (control), 100, and 200 mW was administered concurrently with CED infusions of liposomal rhodamine (LR) or gadolinium-Evans blue-serum albumin conjugated complex (Gd-EBA) at a flow rate of 0.1 µl/min for 1 hour. Line pressures were monitored during the infusions. Rodents were sacrificed immediately following infusion and their brains were harvested, frozen, and serially cryosectioned for histopathologic and volumetric analyses.RESULTS:
Analysis by ANOVA methods demonstrated that co-delivery enhanced volumetric dispersal significantly, with measured volumes of 15.8 ± 0.6 mm(3) for 100 mW compared to 10.0 ± 0.4 mm(3) for its fluid only control and 18.0 ± 0.3 mm(3) for 200 mW compared to 10.3 ± 0.7 mm(3) for its fluid only control. Brains treated with 200 mW co-delivery exhibited thermal lesions, while 100 mW co-deliveries were associated with preservation of brain cytoarchitecture.CONCLUSION:
Both lethal and sub-lethal photothermal hyperthermia substantially increase the rate of volumetric dispersal in a 1 hour CED infusion. This suggests that the FMD co-delivery method could reduce infusion times and the number of catheter insertions into the brain during CED procedures.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Sistemas de Liberación de Medicamentos
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Convección
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Colorantes
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Fibras Ópticas
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Hipertermia Inducida
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Rayos Láser
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Agujas
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Lasers Surg Med
Año:
2013
Tipo del documento:
Article