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[MAPK p38 pathway may be involved in renal function improvement in chronic renal failure rats treated with Jianpi Qinghua decoction].
Ma, Xiao-Hong; Zou, Yun; Zhang, Yue; He, Li-Qun.
Afiliación
  • Ma XH; Department of Nephropathy, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 42(5): 567-72, 2013 Sep.
Article en Zh | MEDLINE | ID: mdl-24167140
ABSTRACT

OBJECTIVE:

To investigate the involvement of MAPK p38 pathway in treatment of chronic renal failure with Jianpi Qinghua Decoction in rats.

METHODS:

Forty SPF SD rats were divided into sham group (n=10),model group (n=10), Jianpi Qinghua group (n=10) and losartan group (n=10). Rat chronic renal failure was induced by 5/6 nephrectomy (Platt method) in model, Jianpi Qinghua and losartan groups, and rats in sham group received sham operation. Jianpi Qinghua decoction (3.9 g 200 g(-1)) or losartan (3.3 g 200 g(-1)) daily were administrated by gavage in Jianpi Qinghua and losartan groups for 60 days, respectively, Rats in sham and model groups were orally administered with saline of the same volume. The serum levels of creatinine and urea nitrogen were measured by biochemical method, the expression of MAPK p38 was detected by Western Blot,and renal pathological changes were observed with hematoxylin-eosin staining.

RESULTS:

Compared to model group,serum creatinine levels after 60d in Jianpi Qinghua and losartan groups were decreased significantly (42.67 ± 5.98 or 40.90 ± 5.07 compared with 60.90 ± 9.54, both P<0.01), the expression of MAPK p38 was significantly down-regulated (0.555 ± 0.004 or 0.587 ± 0.045 compared with 0.930 ± 0.265,both P<0.01) and serum urea nitrogen was also decreased (8.56 ± 0.75 or 7.97 ± 0.86 compared with 8.62 ± 0.62,both P<0.05). The renal pathology in the model group presented glomerular mesangial proliferation,hyperplasia of glomenrulus mesangial cells and interstitial inflammation. Those pathological changes were attenuated significantly in Jianpi Qinghua and losartan groups.

CONCLUSION:

Jianpi Qinghua Decoctions can improve the renal function and renal pathological changes in a rat with chronic renal failure, which may be associated with down-regulation of MAPK p38 immune inflammatory pathways.
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Bases de datos: MEDLINE Medicinas Tradicionales: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas Asunto principal: Medicamentos Herbarios Chinos / Sistema de Señalización de MAP Quinasas / Proteínas Quinasas p38 Activadas por Mitógenos / Fallo Renal Crónico Idioma: Zh Revista: Zhejiang Da Xue Xue Bao Yi Xue Ban Año: 2013 Tipo del documento: Article País de afiliación: China
Buscar en Google
Bases de datos: MEDLINE Medicinas Tradicionales: Medicinas_tradicionales_de_asia / Medicina_china Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas Asunto principal: Medicamentos Herbarios Chinos / Sistema de Señalización de MAP Quinasas / Proteínas Quinasas p38 Activadas por Mitógenos / Fallo Renal Crónico Idioma: Zh Revista: Zhejiang Da Xue Xue Bao Yi Xue Ban Año: 2013 Tipo del documento: Article País de afiliación: China