[Study on chemical components of Aconitum flavum and their inflammatory and analgesic effects].

ABSTRACT

OBJECTIVE: To study the chemical compositions of active site of Aconitum flavum and their pharmacological effects on anti-inflammatory andantalgic. METHODS: Xylene-induced mice ear swollen and egg white-induce toe swollen test were used to assess the anti-inflammatory effect of Aconitum flavum. Acetic acid-induce mice writhing and hot-plate test were used to assess the antalgic effect of Aconitum flavum. Silica gel, ODS and Sephadex LH-20 techniques were employed for isolation and purification the constituents of Aconitum flavum. The chemical compounds were identified by 1H-NMR, 13C-NMR, DEPT, HMBC, HMQC and COSY. RESULTS: When compared with model group, there was significant difference in 30% alcohol active extract group (P < 0.05) in xylene-induce mice ear-swollen test. In the experiment of egg white-induce toe swollen test, 30% alcohol active extract group had great significant difference when compared with the model group (P < 0.01). In acetic acid writhing test, 30% alcohol active extract group had significant analgesic action when compared with model group. In hot plate test, when compared with model group, the analgesic effect of 30% alcohol active extract group (P < 0.05) at 30 and 45 minutes after administration was significanty increased. Fourteen compounds were isolated from the active extract,while seven compounds were identified as sucrose (I), linarin (Ii), beta-sitosterol (III), beta-daucosterol (IV), beta-sitosterol-3, 5, 6-triol (VII), aconitine (V ) and hypaconitine (IV). CONCLUSION: 30% alcohol active extract of Aconitum flavum has anti-inflammatory and antalgic effects. Compounds I, II, IV, VI and VII are isolated for the first time.