Your browser doesn't support javascript.
loading
New compound, 5-O-isoferuloyl-2-deoxy-D-ribono-γ-lacton from Clematis mandshurica: Anti-inflammatory effects in lipopolysaccharide-stimulated BV2 microglial cells.
Dilshara, Matharage Gayani; Lee, Kyoung-Tae; Lee, Chang-Min; Choi, Yung Hyun; Lee, Hak-Ju; Choi, Il-Whan; Kim, Gi-Young.
Afiliación
  • Dilshara MG; Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.
  • Lee KT; Division of Wood Chemistry & Microbiology Department of Forest Products, Korea Forest Research Institute, Republic of Korea.
  • Lee CM; Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.
  • Choi YH; Department of Biochemistry, College of Oriental Medicine, Dong-Eui University, Busan 614-050, Republic of Korea.
  • Lee HJ; Division of Wood Chemistry & Microbiology Department of Forest Products, Korea Forest Research Institute, Republic of Korea.
  • Choi IW; Department of Microbiology, College of Medicine, Inje University, Busan 614-735, Republic of Korea. Electronic address: cihima@inje.ac.kr.
  • Kim GY; Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: immunkim@jejunu.ac.kr.
Int Immunopharmacol ; 24(1): 14-23, 2015 Jan.
Article en En | MEDLINE | ID: mdl-25445966
ABSTRACT
Microglia are main immune cells to exacerbate neural disorders in persistent overactivating. Therefore, it is a good strategy to regulate microglia for the treatment of neural disorders. In the present study, we isolated and characterized a novel compound, 5-O-isoferuloyl-2-deoxy-D-ribono-γ-lacton (5-DRL) from Clematis mandshurica, and evaluated its anti-inflammatory effect in lipopolysaccharide (LPS)-treated BV2 microglial cells. 5-DRL inhibited the expression of LPS-stimulated proinflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2), as well as their regulatory genes inducible NO syntheses (iNOS) and cyclooxygenase-2 (COX-2). 5-DRL also downregulated the LPS-induced DNA-binding activity of nuclear factor-κB (NF-κB) through suppression of the nuclear translocation of the NF-κB subunits, p65 and p50. Consistent with the inhibition of iNOS and COX-2 via NF-κB activity with 5-DRL, an inhibitor of NF-κB, pyrrolidine dithiocarbamate (PDTC), also led to the suppression of LPS-induced iNOS and COX-2 expression. Additionally, 5-DRL corresponding with antioxidants, N-acetylcysteine (NAC) and glutathione (GSH), remarkably inhibited reactive oxygen species (ROS) generation. Both NAC and GSH, thus attenuated the expression of iNOS and COX-2 by suppressing NF-κB activation, indicating that 5-DRL suppresses LPS-induced iNOS and COX-2 expression through downregulation of the ROS-dependent NF-κB signaling pathway. The present study also indicated that 5-DRL suppresses NO and PGE2 production by inducing heme oxygenase-1 (HO-1) via nuclear factor erythroid 2-related factor 2 (Nrf2). Taken together, the present data indicate that 5-DRL attenuates the production of proinflammatory mediators such as NO and PGE2 as well as their regulatory genes in LPS-stimulated BV2 microglial cells by inhibiting ROS-dependent NF-κB activation and stimulating the Nrf2/HO-1 signal pathway. These data may be implicated in the application of 5-DRL in LPS-stimulated inflammatory disease.
Asunto(s)
Palabras clave

Texto completo: 1 Bases de datos: MEDLINE Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas Asunto principal: Antiinflamatorios no Esteroideos / FN-kappa B / Microglía / Clematis / Fitoterapia / Enfermedades del Sistema Nervioso Idioma: En Revista: Int Immunopharmacol Año: 2015 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Métodos Terapéuticos y Terapias MTCI: Terapias_biologicas Asunto principal: Antiinflamatorios no Esteroideos / FN-kappa B / Microglía / Clematis / Fitoterapia / Enfermedades del Sistema Nervioso Idioma: En Revista: Int Immunopharmacol Año: 2015 Tipo del documento: Article