Lipase-catalyzed preparation of phospholipids containing n-3 polyunsaturated fatty acids from soy phospholipids.
J Oleo Sci
; 63(12): 1275-81, 2014.
Article
en En
| MEDLINE
| ID: mdl-25452265
ABSTRACT
To utilize n-3 polyunsaturated fatty acid (PUFA) for a wide range of applications, we prepared phospholipids (PLs) containing PUFAs as constituent fatty acids (PUFA-PLs) via commercially available lipase OF-mediated transacylation with PL from soy (Soy-PL) and ethyl ester of PUFA (PUFA-Et). In a preliminary study to evaluate PUFA-incorporation (wt%) on phosphatidylcholine (PC), we observed that dehydration of Soy-PL is critical. PUFA-incorporation in PLs increased with acyl ratio and time. Finally, maximum PUFA-incorporation (47.1 ± 2.1 wt%) was obtained using the following reaction conditions 2.0 mmol of Soy-PL, a PUFA-Et/Soy-PL acyl ratio of 7, 13 mL of hexane, 2.2 × 10(5) U of lipase OF, 500 rpm of agitation, a temperature of 37°C, and 72 h of reaction time. The analysis of fatty acid composition at the sn-2 position of obtained PL revealed that PUFAs incorporated into Soy-PL localized to the sn-2 position of the PL molecule in spite of using lipase OF whose positional specificity is random for triacylglycerol.
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Bases de datos:
MEDLINE
Asunto principal:
Fosfolípidos
/
Glycine max
/
Ácidos Grasos Omega-3
/
Biocatálisis
/
Lipasa
Idioma:
En
Revista:
J Oleo Sci
Año:
2014
Tipo del documento:
Article