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Enhanced production of protease by Pseudoalteromonas arctica PAMC 21717 via statistical optimization of mineral components and fed-batch fermentation.
Han, Se Jong; Park, Heeyong; Kim, Sunghui; Kim, Dockyu; Park, Ha Ju; Yim, Joung Han.
Afiliación
  • Han SJ; a Division of Polar Life Sciences , Korea Polar Research Institute , Incheon , South Korea.
  • Park H; b Department of Polar Sciences , University of Science and Technology , Incheon , South Korea.
  • Kim S; a Division of Polar Life Sciences , Korea Polar Research Institute , Incheon , South Korea.
  • Kim D; a Division of Polar Life Sciences , Korea Polar Research Institute , Incheon , South Korea.
  • Park HJ; a Division of Polar Life Sciences , Korea Polar Research Institute , Incheon , South Korea.
  • Yim JH; a Division of Polar Life Sciences , Korea Polar Research Institute , Incheon , South Korea.
Prep Biochem Biotechnol ; 46(4): 328-35, 2016 May 18.
Article en En | MEDLINE | ID: mdl-25830556
The objective of this study was to statistically optimize the mineral components of the nutritional medium required for enhancing the production of a cold-active extracellular serine-type protease, W-Pro21717, by the Antarctic bacterium Pseudoalteromonas arctica PAMC 21717. Skim milk was identified as the major efficient inducer. Among the 12 components included in the unoptimized medium, skim milk, NaCl, Na2SO4, Fe(C6H5O7) (ferric citrate), and KCl were determined, by the Plackett-Burman and Box-Behnken design, to have a major effect on W-Pro21717 production. Fed-batch fermentation (5 L scale) using the mineral-optimized medium supplemented with concentrated skim milk (critical medium component) resulted in a W-Pro21717 activity of 53.4 U/L, a 15-fold increment in production over that obtained using unoptimized flask culture conditions. These findings could be applied to scale up the production of cold-active protease.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Péptido Hidrolasas / Pseudoalteromonas / Fermentación / Minerales Tipo de estudio: Prognostic_studies Idioma: En Revista: Prep Biochem Biotechnol Año: 2016 Tipo del documento: Article País de afiliación: Corea del Sur

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Péptido Hidrolasas / Pseudoalteromonas / Fermentación / Minerales Tipo de estudio: Prognostic_studies Idioma: En Revista: Prep Biochem Biotechnol Año: 2016 Tipo del documento: Article País de afiliación: Corea del Sur