Fungal plasma membrane Hâº-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv.
Fitoterapia
; 105: 102-6, 2015 Sep.
Article
en En
| MEDLINE
| ID: mdl-26102180
ABSTRACT
In our ongoing efforts of finding natural fungicides to fight food and feed spoilage during production and storage, the antifungal potential of Ghanaian Uvaria chamae P. Beauv. was investigated, with emphasis on plant metabolites targeting the fungal plasma membrane (PM) H(+)-ATPase. Ethyl acetate extract of U. chamae was subjected to high-resolution fungal PM H(+)-ATPase inhibition screening followed by structural elucidation by high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HPLC-HRMS-SPE-NMR). This led to identification of a series of uncommon o-hydroxybenzylated flavanones and chalcones, i.e., chamanetin (8), isochamanetin (9), isouvaretin (10), uvaretin (11), dichamanetin (12), and diuvaretin (15). Preparative-scale isolation of the active metabolites allowed determination of IC50 values for inhibition of the PM H(+)-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans. These revealed a strong correlation between o-hydroxybenzyl substituents and PM H(+)-ATPase activity, with dichamanetin being the most potent compound, but showing moderate activity in the fungal growth inhibition assays.
Palabras clave
Chamanetin (PubChem CID: 5315801); Dichamanetin (PubChem CID: 181193); Diuvaretin (PubChem CID: 3085222); Fungal plasma membrane H(+)-ATPase; HPLCHRMSSPENMR; High-resolution screening; Isochamanetin (PubChem CID: 21721817); Pinocembrin (PubChem CID: 68071); Uvaretin (PubChem CID: 73447); Uvaria chamae
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ATPasas de Translocación de Protón
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Uvaria
/
Flavanonas
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Chalconas
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Antifúngicos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Fitoterapia
Año:
2015
Tipo del documento:
Article