Antithrombotic activity of Vitis labrusca extract on rat platelet aggregation.
Blood Coagul Fibrinolysis
; 27(2): 141-6, 2016 Mar.
Article
en En
| MEDLINE
| ID: mdl-26340455
ABSTRACT
Vitis labrusca is a grapevine that has antioxidant, neuroprotective, hepatoprotective, and anticarcinogenic activity. However, the antithrombotic effect of Vitis labrusca leaves on platelets is yet to be ascertained. We investigated the inhibitory effect of V. labrusca leaf extract (VLE) on platelet aggregation in vitro and ex vivo. The thromboxane B2 (TXB2) and serotonin concentrations were measured by ELISA. The flavonoids content was measured by ultraperformance liquid chromatography (UPLC). The antithrombotic activity of VLE was evaluated using various agonists in vitro. VLE strongly inhibited adenosine diphosphate (ADP)-induced platelet aggregation. In rats, VLE treatment (100âmg/kg) reduced ADP-stimulated platelet aggregation, without affecting tail bleeding and coagulation time. Moreover, VLE significantly suppressed TXB2 and serotonin secretion. UPLC analysis indicated that VLE contains quercetin, isorhamnetin, and rutin. Our results indicate that VLE possesses antiplatelet activity via the suppression of TXB2 and serotonin, without affecting bleeding. Further, we identified the flavonoids present in VLE. Thus, VLE may be a potential agent for the prevention of cardiovascular diseases.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Flavonoides
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Plaquetas
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Inhibidores de Agregación Plaquetaria
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Extractos Vegetales
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Agregación Plaquetaria
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Vitis
Idioma:
En
Revista:
Blood Coagul Fibrinolysis
Año:
2016
Tipo del documento:
Article