Enhancement of radiosensitivity of lung adenocarcinoma using a decoction from the Fuzhengzengxiao formula.
J Tradit Chin Med
; 35(6): 690-6, 2015 Dec.
Article
en En
| MEDLINE
| ID: mdl-26742316
ABSTRACT
OBJECTIVE:
To study the effects of a decoction of Fuzhengzengxiao formula on lung adenocarcinoma regarding the inflammatory protein S100A9 known to enhance cancer cell sensitivity.METHODS:
A nude mouse model of human lung adenocarcinoma was established. The mice were randomly divided into four groups using the random number tablemethod:
Group I, control; Group II, treatment with a decoction of the Fuzhengzengxiao formula alone; Group III, treatment with radiotherapy alone; and Group IV, treatment with radiotherapy plus a decoction of Fuzhengzengxiao formula. When the tumor body was 1 cm3 in diameter, the tumor bearing mice in Groups III and IV were irradiated at a single dose of 10 Gy and the tumor inhibition rate was evaluated. The expression of S100A9 was determined using Western blotting and q-PCR (Real-time Quantitative PCR Detecting System). The sensitivity of cells containing RNAi S100A9 to radiotherapy was evaluated using the Click multiple target model,and the cell cycle was analyzed using flow cytometry.RESULTS:
Relative to the control group, the expression of S100A9 in the tumors in each treatment group was decreased, especially in Group IV. The sensitizing enhancement ratio (SER) Dq was > 1 after RNAi S100A9; it decreased the surviving fraction after a 2 Gy dose exposure,and also the D0 and Dq of the tumor cells; in addition, the radiosensitivity of G2/M cells was significantly increased.CONCLUSION:
The decoction of the Fuzhengzengxiao formula downregulated the expression of S100A9 in lung adenocarcinoma cells.
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Bases de datos:
MEDLINE
Medicinas Tradicionales:
Medicinas_tradicionales_de_asia
/
Medicina_china
Asunto principal:
Medicamentos Herbarios Chinos
/
Adenocarcinoma
/
Neoplasias Pulmonares
Idioma:
En
Revista:
J Tradit Chin Med
Año:
2015
Tipo del documento:
Article