The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr.
Molecules
; 21(1): 90, 2016 Jan 14.
Article
en En
| MEDLINE
| ID: mdl-26784151
ABSTRACT
The amount of secondary metabolites in plants can be enhanced or reduced by various external factors. In this study, the effect of strontium ions on the production of phytoestrogens in soybeans was investigated. The plants were treated with Hoagland's solution, modified with Sr(2+) with concentrations ranging from 0.5 to 3.0 mM, and were grown for 14 days in hydroponic cultivation. After harvest, soybean plants were separated into roots and shoots, dried, and pulverized. The plant material was extracted with methanol and hydrolyzed. Phytoestrogens were quantified by HPLC. The significant increase in the concentration of the compounds of interest was observed for all tested concentrations of strontium ions when compared to control. Sr(2+) at a concentration of 2 mM was the strongest elicitor, and the amount of phytoestrogens in plant increased ca. 2.70, 1.92, 3.77 and 2.88-fold, for daidzein, coumestrol, genistein and formononetin, respectively. Moreover, no cytotoxic effects were observed in HepG2 liver cell models after treatment with extracts from 2 mM Sr(2+)-stressed soybean plants when compared to extracts from non-stressed plants. Our results indicate that the addition of strontium ions to the culture media may be used to functionalize soybean plants with enhanced phytoestrogen content.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Glycine max
/
Estroncio
/
Extractos Vegetales
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Brotes de la Planta
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Raíces de Plantas
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Fitoestrógenos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Molecules
Año:
2016
Tipo del documento:
Article
País de afiliación:
Polonia